Journal: Nanomaterials

Article Title: Development and Biocompatibility Evaluation of Photocatalytic TiO 2 /Reduced Graphene Oxide-Based Nanoparticles Designed for Self-Cleaning Purposes

doi: 10.3390/nano7090279

Figure Lengend Snippet: Representative images of phase contrast microscopy revealing cellular morphology of CCD 1070Sk ( a ) and MRC-5 ( b ) fibroblasts exposed to 100 and 200 μg/mL of two TiO 2 /RGO nanocomposites samples (A and B) and commercially available TiO 2 NPs (P25) for 24 and 48 h. Scale bar: 100 μm.

Article Snippet: Normal human lung (MRC-5 cell line, ATCC Cat. No. CCL-171) and skin (CCD 1070Sk cell line, ATCC Cat. No. CRL-2091) fibroblasts were cultured in complete Eagle’s minimum essential medium (MEM; Gibco/Invitrogen, Carlsbad, CA, USA) with an addition of 10% fetal bovine serum (FBS; Gibco/Invitrogen, Carlsbad, CA, USA) at 37 °C, in a 5% CO 2 humidified atmosphere.

Techniques: Microscopy

Journal: Nanomaterials

Article Title: Development and Biocompatibility Evaluation of Photocatalytic TiO 2 /Reduced Graphene Oxide-Based Nanoparticles Designed for Self-Cleaning Purposes

doi: 10.3390/nano7090279

Figure Lengend Snippet: Lysosomes profile in skin (CCD 1070Sk) and lung (MRC-5) fibroblasts after 48 h exposure to different concentrations (100 and 200 μg/mL) of the two TiO 2 /RGO nanocomposites samples (A and B) and commercially available TiO 2 (P25). Lysosomes (green) staining was done with LysoTracker Green DND-26 and nuclei (blue) were counterstained with Hoechst 33342. Scale bar: 100 μm.

Article Snippet: Normal human lung (MRC-5 cell line, ATCC Cat. No. CCL-171) and skin (CCD 1070Sk cell line, ATCC Cat. No. CRL-2091) fibroblasts were cultured in complete Eagle’s minimum essential medium (MEM; Gibco/Invitrogen, Carlsbad, CA, USA) with an addition of 10% fetal bovine serum (FBS; Gibco/Invitrogen, Carlsbad, CA, USA) at 37 °C, in a 5% CO 2 humidified atmosphere.

Techniques: Staining

Journal: Nucleic Acids Research

Article Title: Assessment of gene expression in many samples using vertical arrays

doi: 10.1093/nar/gkn263

Figure Lengend Snippet: Scatter plots for gene AA428473, which maps to Nuclear receptor subfamily 1, group D, member 2 (NR1D2). On the x -axis, 1–96 correspond to replicate measurements made at t = 20 min after re-feeding serum-starved fibroblasts, and 97–192 correspond to measurements made at t = 240 min, except for OPN28, for which twice as many measurements were made at all time points. The vertical axes are the log2( I t / I t = 0 ) and the blue and red lines pass through the median values for the 20 and 240 min, respectively. Four-fold down-regulation of NR1D2 is implied at t = 240 by two different LCRs, d9 and h8.

Article Snippet: Normal human diploid fibroblasts (cell line ATCC CRL 2091) were deprived of serum for 48 h and serum was reintroduced as described by Iyer et al . ( ).

Techniques:

Journal: Nucleic Acids Research

Article Title: Assessment of gene expression in many samples using vertical arrays

doi: 10.1093/nar/gkn263

Figure Lengend Snippet: Boxplot summary of results from vertical array measurements for all genes tested, showing the log2 of the ratio of intensities measured at t = 0 and t = 240 min after re-feeding serum to serum-starved fibroblasts. The vertical lines near the center of each box correspond to the median. The whiskers extend to the most extreme data point, which is not more than 1.5 times the interquartile range from the box. The red dotted vertical lines show the position of a 2-fold change.

Article Snippet: Normal human diploid fibroblasts (cell line ATCC CRL 2091) were deprived of serum for 48 h and serum was reintroduced as described by Iyer et al . ( ).

Techniques: